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Lentivirus Production

Lentivirus Production Service

Lentivirus Production Service

Establishment of standardized manufacturing methods using a third-generation lentivirus packaging system

Provides highly concentrated/highly efficient recombinant lentivirus vectors for in vitro and in vivo experiments

Titer measurement by confirmation of lentivirus number (copies/mL) through qRT-PCR, and infection rate analysis by direct detection of the expression of target gene in transformed cells.

Using company-owned lentivirus vectors and lentivirus vectors provided by the client

Lentivirus vector of Pharos Vaccine

pPVLV(s)

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pPVLV(s)

pPVLV(d)

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pPVLV(d)

service description

Service Exported titre(TU/ml) Volume
Regular-scale production of Lentivirus 1 x 10 7-8 1 mL
Large-scale production of Lentivirus 1 x 10 8-9 0.5 mL
Control GFP Lentivirus 1 x 10 8 0.2 mL

Manufacturing method of customized Lentivirus

▪Using lentivirus vectors provided by the sponsor

Plasmid gene map and gene sequence

Plasmid gene 1-5 μg

Prepare endotoxin-free plasmids and transform them into packaging cell line (HEK293 Cell Line), harvest lentivirus and provide it after measurement of concentration and titer.

▪Using company-owned lentivirus vectors

Choose company-owned lentivirus vector and designate target gene

Plasmid gene 1-5 μg or genetic sequence information containing target gene

Clone target gene into lentivirus vector, prepare endotoxin-free plasmids and transform them into packaging cell line (HEK293 Cell Line), harvest lentivirus. and provide it after measurement of concentration and titer.

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